Hydrogen peroxide assay by using enhanced chemiluminescence of the luminol-H202--horseradish peroxidase system: Comparative studies
نویسندگان
چکیده
p-Iodophenol, p-coumaric acid, phenol and aniline are enhancers of the luminol-H202-horseradish peroxidase chemiluminescence. These enhanced and unenhanced chemiluminescences were used to assay low concentrations of hydrogen peroxide. The curves of cherniluminescence intensity maxima against hydrogen peroxide concentration were fitted to third order polynomial regressions, which permit a very good approach to the experimental results. Double the sensitivity (RSD 5 4.3%) and a slightly lower detection limit (e.g. 0.08 pM with p-coumaric acid of 0.18 pM without enhancer) were obtained with enhanced chemiluminescence than with unenhanced chemiluminescence.
منابع مشابه
Enhanced chemiluminescence biosensor for the determination of phenolic compounds and hydrogen peroxide
The enhanced chemiluminescence (CL) reaction of the luminol±H2O2±horseradish peroxidase (HRP) system with immobilized HRP using microencapsulation in a sol±gel matrix has been used to develop a biosensor for p-iodophenol, p-coumaric acid, 2-naphthol and hydrogen peroxide. The detection limits obtained for p-iodophenol, p-coumaric acid and 2-naphthol were 0.83 mM, 15 and 48 nM, respectively. Dir...
متن کاملApplication of 4-iodophenol-enhanced luminol chemiluminescence to direct detection of horseradish peroxidase encapsulated in liposomes.
4-Iodophenol was applied to an enhancer in the direct detection of horseradish peroxidase (HRP) encapsulated in liposomes by using luminol chemiluminescence (CL). Luminol, 4-iodophenol and hydrogen peroxide permeate into the inner phase of liposomes containing HRP, resulting in the progress of 4-iodophenol-enhanced luminol CL catalyzed by HRP in liposomes. The CL intensity observed in liposomes...
متن کاملDirect determination of plasma endothelin-I by chemiluminescence enzyme immunoassay.
A highly sensitive sandwich-type chemiluminescence enzyme immunoassay for plasma endothelin-I (ET-1), involving no extraction steps, has been developed. Two populations of polyclonal antibodies were used in the present study: One is specific to the C-terminus of the endothelin family of peptides; the other, a Fab' fragment against the N-terminal core region of ET-1, is coupled with horseradish ...
متن کاملImmunodot detection of Escherichia coli heat-stable enterotoxin b by using enhanced chemiluminescence reaction.
An indirect immunodot assay with rabbit antibodies raised against purified heat-stable enterotoxin type b (STb) and with a Western blotting (immunoblotting) detection system (ECL; Amersham International plc, Amersham, United Kingdom) was developed for the detection of STb toxin. Culture supernatants of 62 Escherichia coli isolates from pigs with diarrhea were blotted onto nitrocellulose and inc...
متن کاملEnhanced chemiluminescence of the luminol-hydrogen peroxide system by colloidal cupric oxide nanoparticles as peroxidase mimic.
As a peroxidase mimic, cupric oxide nanoparticles were found to enhance the chemiluminescence (CL) of luminol-H(2)O(2) system up to 400 folds. The CL spectra and radical scavengers were conducted to investigate the possible CL enhancement mechanism. It was suggested that the enhanced CL could be attributed to the peroxidase-like activity of CuO nanoparticles, which effectively catalyzed the dec...
متن کامل